Полученные в результате исследования данные показали, что сверхэкспрессия гена VaCPK13 не влияла на устойчивость клеток V. amurensis к температурному, осмотическому и солевому стрессам. Полученные результаты свидетельствуют, что ген VaCPK13 не является сильным позитивным регулятором ответа растений на проанализированные абиотическиие стрессы.
Са 2+-зависимые протеинкиназы, Vitis amurensis, абиотический стресс, клеточные культуры растений, Cа
1. FAO (Food, Agriculture Organization of the UnitedNations) // FAO production yearbook. - Rome, FAO. - 2004.
2. Трансгенные растения, толерантные к абиотическим стрессам / Я.С. Колодяжная, Н.К. Куцоконь, Б.А. Левенко [и др.] // Цитология и генетика. - 2009. - Т. 2. - С. 72-93.
3. Al Khatib K., Paulsen G.M. High temperature effects on photosynthetic processes in temperate and tropical cereals // Crop Sci. Soc. Amer. - 1999. - V. 39 - P. 119-125.
4. McAinsh M.R. and Pittman J.K. Shaping the calcium signature // New Phytol. - 2009. - V.181. -P. 275-294.
5. DeFalco T.A., Bender K.W. and Snedden W.A. Breaking the code: Ca2+ sensors in plant signaling // Biochem. J. - 2010. - V. 425. - P.27-40.
6. Медведев С.С. Кальциевая сигнальная система растений. Физиология растений. - 2005. -№ 52. - Т. 1. - С. 1-24.
7. Das R., Pandey G.K. Expressional analysis and role of calcium regulated kinases in abiotic stress signaling. Curr Genomics. - 2010. - V. 11. - P. 2-13.
8. Boudsocq M., Sheen J. CDPKs in immune and stress signaling // Trends Plant Sci. - 2013. - V.1. -P. 30-40.
9. Dubrovina A.S., Kiselev K.V., & Khristenko V.S. Expression of calcium-dependent protein kinase (CDPK) genes under abiotic stress conditions in wild-growing grapevine Vitis amurensis // J. Plant Physiol. - 2013. - V.170. - P.1491-1500.
10. Kiselev K.V., Dubrovina A.S., & Bulgakov V.P. Phenylalanine ammonia-lyase and stilbene synthase gene expression in rolB transgenic cell cultures of Vitis amurensis // Appl Microbiol Biotechnol. -2009. - V. 82. - P. 647-655.
11. pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants / T. Tzfira, G.W. Tian, B. Lacroix [et al.] // Plant Mol Biol. - 2005. - V. 57. -P. 503-516.
12. The rolB gene-induced overproduction of resveratrol in Vitis amurensis transformed cells / K.V. Kiselev, A.S. Dubrovina, M.V. Veselova [et al.] // J Biotechnol. - 2007. - V.128. - P. 681-692.
13. Bekesiova I., Nap J., M.Iynarova L. isolation of high quality DNA and RNA from leaves of the carnivorous plant Drosera rotundifolia // Plant Mol Biol Rep. - 1999. - V. 17. - P. 269-77.
14. Enhanced resveratrol accumulation in rolB transgenic cultures of Vitis amurensis correlates with unusual changes in CDPK gene expression / A.S. Dubrovina, K.V. Kiselev, M.V. Veselova [et al.] // J. Plant Physiol. - 2009. - V.166 - P.1194-206.
15. An overview of real-time quantitative PCR: applications to quantify cytokine gene expression / A. Giulietti, L. Overbergh, D. Valckx [et al.] // Methods. - 2001. - V. 25 - P. 386-401.
16. Structure and expression profiling of a novel calcium-dependent protein kinase gene, CDPK3a, in leaves, stems, grapes, and cell cultures of wild-growing grapevine Vitis amurensis Rupr / K.V. Kiselev, A.S. Dubrovina, O.A. Shumakova [et al.] // Plant Cell Rep. - 2013. - V. 3. - P. 431-42.
17. The Evolutionary History and Diverse Physiological Roles of the Grapevine Calcium-Dependent Protein Kinase Gene Family / Chen Fei, Fasoli Marianna, Tornielli Giovanni Battista [et al.] // PLoS ONE. - 2013. - Т. 12. - P. 808-818.
18. Genome-wide identification of the Rice Calcium-dependent Protein Kinase and its Closely Related Kinase Gene Families: Comprehensive Analysis of the CDPKs Gene Family in Rice / Asano Takayuki, Tanaka Naoki, Yang Guangxiao [et al.] // Plant Cell Physiol. - 2005. - V. 2. - P. 356-366.
19. Expression profile of calcium-dependent protein kinase (CDPKs) genes during the whole lifespan and under phytohormone treatment conditions in rice (Oryza sativa L. ssp. indica) / Shuifeng Ye, Lei Wang, Weibo Xie [et al.] // Plant Mol Biol. - 2009. - V. 70. - P. 311-325.
20. Genome-wide identification, classification, and expression analysis of CDPK and its closely related gene families in poplar (Populus trichocarpa) / Ran Zuo, Ruibo Hu, Guohua Chai [et al.] // Mol Biol Rep. - 2013. - V. 40. - P. 2645-62.
