The obtained as research result data showed that the VaCPK13gene overexpression did not influence on the stability of the V. amurensis cells to the temperature, osmotic and salt stress. The obtained results prove that the gene VaCPK13 is not a strong positive regulator of the plant response to the analyzed abiotic stress.
2+dependent protein kinase, Vitis amurensis, abiotic stress, plantcell cultures
1. FAO (Food, Agriculture Organization of the UnitedNations) // FAO production yearbook. - Rome, FAO. - 2004.
2. Transgennye rasteniya, tolerantnye k abioticheskim stressam / Ya.S. Kolodyazhnaya, N.K. Kucokon', B.A. Levenko [i dr.] // Citologiya i genetika. - 2009. - T. 2. - S. 72-93.
3. Al Khatib K., Paulsen G.M. High temperature effects on photosynthetic processes in temperate and tropical cereals // Crop Sci. Soc. Amer. - 1999. - V. 39 - P. 119-125.
4. McAinsh M.R. and Pittman J.K. Shaping the calcium signature // New Phytol. - 2009. - V.181. -P. 275-294.
5. DeFalco T.A., Bender K.W. and Snedden W.A. Breaking the code: Ca2+ sensors in plant signaling // Biochem. J. - 2010. - V. 425. - P.27-40.
6. Medvedev S.S. Kal'cievaya signal'naya sistema rasteniy. Fiziologiya rasteniy. - 2005. -№ 52. - T. 1. - S. 1-24.
7. Das R., Pandey G.K. Expressional analysis and role of calcium regulated kinases in abiotic stress signaling. Curr Genomics. - 2010. - V. 11. - P. 2-13.
8. Boudsocq M., Sheen J. CDPKs in immune and stress signaling // Trends Plant Sci. - 2013. - V.1. -P. 30-40.
9. Dubrovina A.S., Kiselev K.V., & Khristenko V.S. Expression of calcium-dependent protein kinase (CDPK) genes under abiotic stress conditions in wild-growing grapevine Vitis amurensis // J. Plant Physiol. - 2013. - V.170. - P.1491-1500.
10. Kiselev K.V., Dubrovina A.S., & Bulgakov V.P. Phenylalanine ammonia-lyase and stilbene synthase gene expression in rolB transgenic cell cultures of Vitis amurensis // Appl Microbiol Biotechnol. -2009. - V. 82. - P. 647-655.
11. pSAT vectors: a modular series of plasmids for autofluorescent protein tagging and expression of multiple genes in plants / T. Tzfira, G.W. Tian, B. Lacroix [et al.] // Plant Mol Biol. - 2005. - V. 57. -P. 503-516.
12. The rolB gene-induced overproduction of resveratrol in Vitis amurensis transformed cells / K.V. Kiselev, A.S. Dubrovina, M.V. Veselova [et al.] // J Biotechnol. - 2007. - V.128. - P. 681-692.
13. Bekesiova I., Nap J., M.Iynarova L. isolation of high quality DNA and RNA from leaves of the carnivorous plant Drosera rotundifolia // Plant Mol Biol Rep. - 1999. - V. 17. - P. 269-77.
14. Enhanced resveratrol accumulation in rolB transgenic cultures of Vitis amurensis correlates with unusual changes in CDPK gene expression / A.S. Dubrovina, K.V. Kiselev, M.V. Veselova [et al.] // J. Plant Physiol. - 2009. - V.166 - P.1194-206.
15. An overview of real-time quantitative PCR: applications to quantify cytokine gene expression / A. Giulietti, L. Overbergh, D. Valckx [et al.] // Methods. - 2001. - V. 25 - P. 386-401.
16. Structure and expression profiling of a novel calcium-dependent protein kinase gene, CDPK3a, in leaves, stems, grapes, and cell cultures of wild-growing grapevine Vitis amurensis Rupr / K.V. Kiselev, A.S. Dubrovina, O.A. Shumakova [et al.] // Plant Cell Rep. - 2013. - V. 3. - P. 431-42.
17. The Evolutionary History and Diverse Physiological Roles of the Grapevine Calcium-Dependent Protein Kinase Gene Family / Chen Fei, Fasoli Marianna, Tornielli Giovanni Battista [et al.] // PLoS ONE. - 2013. - T. 12. - P. 808-818.
18. Genome-wide identification of the Rice Calcium-dependent Protein Kinase and its Closely Related Kinase Gene Families: Comprehensive Analysis of the CDPKs Gene Family in Rice / Asano Takayuki, Tanaka Naoki, Yang Guangxiao [et al.] // Plant Cell Physiol. - 2005. - V. 2. - P. 356-366.
19. Expression profile of calcium-dependent protein kinase (CDPKs) genes during the whole lifespan and under phytohormone treatment conditions in rice (Oryza sativa L. ssp. indica) / Shuifeng Ye, Lei Wang, Weibo Xie [et al.] // Plant Mol Biol. - 2009. - V. 70. - P. 311-325.
20. Genome-wide identification, classification, and expression analysis of CDPK and its closely related gene families in poplar (Populus trichocarpa) / Ran Zuo, Ruibo Hu, Guohua Chai [et al.] // Mol Biol Rep. - 2013. - V. 40. - P. 2645-62.
