Krasnodar, Russian Federation
Krasnodar, Russian Federation
Krasnodar, Russian Federation
632
The purpose of research is to study the spread of phytoplasma infection in the vineyards of the Krasnodar Region using the PCR method. The object of the study is grape plants of various varieties with external symptoms of phytoplasma infection. The material for total DNA extraction was symptomatic leaves (their veins and petioles) collected from plantings of Shiraz, Chardonnay, Cabernet Sauvignon, Saperavi, Mizar, Moldova, Augustine, Pinot Blanc, Riesling, and Aligote varieties. DNA extraction was carried out using a buffer based on the cationic detergent CTAB (cetyltrimethylammonium bromide), according to the protocol recommended by EPPO (European plant protection organization) for the isolation of phytoplasmas from grape plant material. Sampling was carried out in industrial vineyards of the Krasnodar Region in 9 different geographical locations of the Novorossiysk, Temryuk and Anapa Regions. DNA was isolated from symptomatic leaves according to the protocol recommended by EPPO (European plant protection organization). Determination of the presence of phytoplasma infection was carried out by real-time PCR on a Quant Studio 5 amplifier using a commercial kit designed to detect phytoplasmas – Candidatus Phytoplasma solani and Candidatus Phytoplasma vitis (Synthol) and a test system recommended by EPPO. Using a kit produced by Synthol, 239 samples were analyzed, including 12 rechecked with markers recommended by EPPO. As a result of the analysis, it was found that DNA of Candidatus Phytoplasma solani was present in 113 samples studied. DNA from the phytoplasma Candidatus Phytoplasma vitis, which causes golden yellowing of leaves, was not detected in any of the analyzed samples.
Candidatus Phytoplasma solani, Candidatus Phytoplasma vitis, Real time PCR
1. Lee I.M., Zhao Y., Davis R.E. Prospects of multiple gene-based systems for differentiation and classification of phytoplasmas // Phyto-plasmas: Genomes, plant hosts and vectors. Wallingford: CABI, 2010. P. 51–63.
2. Grapevine phytoplasmas / E. Angelini [et al.] // Phytoplasmas: Plant Pathogenic Bacteria – I, Characterisation and Epidemiology of Phyto-plasma – Associated Diseases. Springer, Singapore, 2018. P. 123–152.
3. Identification of phytoplasmas associated with grapevine ‘bois noir’ and flavescence dorée in inter-row groundcover vegetation used for green manure in Franciacorta vineyards / A. Moussa [et al.] // Journal of Plant Pathology. 2023. Vol. 1. P. 1–9.
4. Geographical and Temporal Diversity of ‘Can-didatus Phytoplasma solani' in Wine-Growing Regions in Slovenia and Austria / N. Mehle [et al.] // Frontiers in Plant Science. 2022. Vol. 13. P. 889675.
5. Alma A., Lessio F., Nickel H. Insects as phyto¬plasma vectors: Ecological and epidemiological aspects // Phytoplasmas: Plant Pathogenic Bacteria – II, Transmission and Management of Phytoplasma – Associated Diseases. Springer: Singapore, 2019. P. 1–25.
6. Porotikova E., Yurchenko E., Vinogradova S. First Report of ‘Candidatus Phytoplasma solani’ Associated with Bois Noir on Grapevine (Vitis vinifera) in Krasnodar Region of Russia // Plant Disease. 2019. Vol.104. P. 227.
7. Revised classification scheme of phytoplas-mas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences / I.M. Lee [et al.] // International journal of syste-matic bacteriology. 1998. Vol. 48. P. 1153–1169.
8. Gnezdilov V.M., Orlov O.V. First Record of Scaphoideus titanus Ball, 1932 (Hemiptera, Auchenorrhyncha: Cicadellidae: Deltocephali-nae), a Vector of flavescence doreé of Grapevine, from Ciscaucasia // Entomological Review. 2022. Vol. 102. P. 108–109.
9. Martynov V.V., Nikulina T.V. Scaphoideus titanus Ball, 1932 (Hemiptera: Cicadellidae) – novyy invazivnyy vreditel' vinograda na territorii Donbassa // Biologiya rasteniy i sadovodstvo: teoriya, innovacii. 2019. № 153. S. 49–57.
10. Hamaeva B.B., Bondarenko G.N., Radionovskaya Ya.E. Izuchenie vidovogo sostava cikadovyh (Auchenorrhyncha) v ampelocenozah Respubliki Krym // Fitosanitariya. Karantin rasteniy. 2022. № 3. S. 45–52.
11. European and Mediterranean Plant Protection Organization. PM 7/079 (2) Grapevine flave-scence doree phytoplasma // Bulletin OEPP/ EPPO Bulletin. 2016. Vol. 46 (1). P. 78–93.
