One of dangerous parasites of the honey bee is the wax moth. Its larvae cause essential damage to bee families. The most perspective is biological method of fight against the wax moth as it guaran-tees the safest impact on the honey bee, having thus pernicious effect on the larvae of the moth. In the department of microbiology in FSBSE FEZRVI the biological preparation Antigallerin received on the basis of the strain of Bacillus thuringiensis 846-Bt was approved. The purpose of the research was studying the toxicity of the experimental preparation "Antigallerin" for bees. The effect of the preparation "Antigallerin» was investigated in using three dos-es. The results of research on studying of acute toxicity showed that single feeding of Antigallerin with the forage in minimum, average and maximum doses had no negative impact on experimental bees’ behavior and did not cause their death. When studying chronic contact toxicity symptoms of intox-ication were not revealed in the minimum doses and in average and maximum the death of bees was observed in 20 and 100 % of the cases respec-tively. The data received during the researches of bacterial preparations on toxicity should be applied in the course of justification of actions for the wax moth control in climatic conditions of the Amur Re-gion for the purpose of the definition of the mini-mum dose of the preparation, safe to the honey bee, but possessing maximum efficiency.
Bacillus thuringiensis, Antigallerin, wax moth, larvae, biological control, honey bee, acute toxicity, chronic toxicity
1. Aktivnyy shtamm Bacillus thuringiensis - producent belka δ-endotoksina / I.E. Pa-ramonova, M.V. Fomenko, D.S. Balpanov [i dr.] // Vestnik Evraziyskogo nacional'- nogo universiteta im. L.N. Gumileva. - 2013. - № 6. - S. 262-269.
2. Bioinsekticid dlya bor'by s bol'shoy vos-kovoy mol'yu: pat. 2603271 Rossiyskaya Fe-deraciya, MPK MPK A01N63/00 / I.S. Shul'-ga, V.A. Ryabuha, N.N.Shul'ga, L.A. Lavrushi-na, V.A. Konoplev, A.V. Kurazeeva; zayavi-tel' i patentoobladatel' FGBNU Dal' ZNIVI. - № 2015130407; zayavl. 22.07.2015.
3. Biologicheskie metody bor'by s voskovoy mol'yu / A.S. Osokina, L.M. Kolbina [i dr.] // Biotehnologicheskie aspekty razvitiya so- vremennogo pchelovodstva: mat-ly II Mezhdu- nar. nauch.-prakt. konf. - 2015. - S. 119-122.
4. Biopreparat «Antigallerin» dlya bor'by s voskovoy mol'yu / A.M. Kuz'menko, V.A. Se-rebryakova [i dr.] // Problemy veterinar-noy mediciny i zooekologii Rossiyskogo i Aziatsko-Tihookeanskogo regionov: mat-ly pervoy mezhdunar. nauch.-prakt. konf. / Ros. akad. s.-h. nauk, GNU Dal'ZNIVI, Min-vo sel. hoz.-va Amur. obl. - Blagoveschensk, 2012. - S. 15-17.
5. Vydelenie i otbor vysokovirulentnyh kul'tur Bacillus thuringiensis var. galleriae: metod. rekomendacii / A.G. Kol'chevskiy [i dr.]; VNII zaschity rasteniy. - Lenin-grad, 1987. - 21 s.
6. Izuchenie insekticidnoy aktivnosti prepa-rata na osnove shtamma Bacillus thuringiensis RCAM 00045 pri ispol'zovanii razlichnyh sposobov konservacii / V.A. Konoplev, A.V. Kurazeeva, L.A. Lavrushina [i dr.] // Vestnik KrasGAU. - 2015. - № 12 (10). - S. 191-194.
7. Klochko R.T., Luganskiy S.N., Kotova A.A. Bol'shaya voskovaya mol' // Pchelovodstvo. - 2012. - № 2. - S. 24-26.
8. Konovalova T.V. Borites' s voskovoy mol'yu // Pchelovodstvo. - 1995. - № 1. - S. 25-26.
9. Metodicheskie rekomendacii po izucheniyu toksicheskogo deystviya pesticidov i bio-preparatov na pchel / V.F. Titov, N.A. Vas'kov, I.M. Stolbov [i dr.]. - M., 1989. - 21 s.
10. Metodicheskie ukazaniya po opredeleniyu toksicheskih svoystv preparatov, prime-nyaemyh v veterinarii i zhivotnovodstve. Veterinarnye preparaty: sprav. / sost. L.P. Malinin [i dr.]. - M.: Agropromizdat, 1988. - S. 239-289.
11. Ocenka biologicheskoy aktivnosti nekoto- ryh kollekcionnyh shtammov Bacillus thuringiensis po otnosheniyu k lichinkam che-shuekrylyh na model'nom ob'ekte Galleria Mellonella / M.V. Lozovskaya, I.I. Novikova, I.V. Boykova [i dr.] // Estestvennye nauki. - 2014. - № 4 (49). - S. 95-100.
12. Shtamm bakteriy Bacillus thuringiensis dlya polucheniya bioinsekticida dlya bor'by s bol'shoy voskovoy mol'yu: pat. 2453595 Rossiyskaya Federaciya, MPK S12N 1/20 / N.E. Gorkovenko, Yu.A. Makarov, V.A Sereb-ryakova; zayavitel' i patentoobladatel' Dal'nevostochnyy zon. nauch.-issled. vete-rinar. in-t. - № 2011110498/10; zayavl. 18.03.2011; opubl. 20.06.2012, Byul. № 17. - 4 s.
13. Ekologiya medonosnoy pchely, Apis mellifera L. (vliyanie pesticidov i drugih antropo-gennyh faktorov): ucheb. posobie / L.A. Osinceva; Novosib. gos. agrar. un-t. - No-vosibirsk, 1999. - 42 s.
