By using methods to identify regions of nucleolar organizers (Adao) with silver nitrate, the studied parameters, Adao in the cells of the renal tubules after partial nephrectomy using for suturing the surgical wound of the kidney thread «Alloplant» were studied. Studies have shown that Adao in the nuclei of cells of renal tubules of rabbits have a rounded or close to it in the shape, and the number of Adao is in the range from one to four. It was es-tablished that the greatest number of Adao in the nuclei of kidney tubular cells identified at the third day in a zone close to the scar (2.73±0.08) and lowest at the twelfth day was away from the rumen (1.80±0.04). The maximum increase of the total area of Adao recorded on the third day in the area of damage (4.76±0.18 µm2) and away from it (2.85±0.09 µm2), then there was a decrease of this parameter in the zone close to the scar to the fif-teenth day (2.93±0.10 µm2), and in the distance from it to the twelfth day (2.04±0.07 µm2). During the eighteenth and sixtieth days the value of this preoperative parameter approach, in the zone close to the surgical scar, and remote from him was in-vestigated. We obtained data on the dynamics of changes in the number of Adao in the nuclei of cells of renal tubules after partial nephrectomy co-incided with the dynamics of change of the total ar-ea of Adao. The research results indicated that in the thread «Alloplant» for suturing the surgical wound of the kidney after performing nephrectomy in the renal tubules, the change in the number and total area of Adao depending on proximity to the surgical scar that, in our opinion, reflected the dif-ferent proliferative potential of the cells of the renal tubules and the state of cellular differentiation.
rabbits, kidneys, renal tubules, nu-cleolus organizers, ribosomal RNA, alloplant, re-generation
1. Aktivaciya yadryshkovyh organizatorov pri kul'tivirovanii myshinyh embrional'nyh stvolovyh kletok linii RL in vitro / E.R. Kunafina [i dr.] // Ontogenez. - 2005. - № 36 (2). - S. 102-109.
2. Argirofil'nye belki oblastey yadryshko-vyh organizatorov - markery skorosti kletochnoy proliferacii / N.T. Rayhlin [i dr.] // Arhiv patologii. - 2006. - T. 68, № 3. - C. 47-51.
3. Demin S.Yu., Stefanova V.N. Differen-ciaciya interfaznyh yadrysh-kovyh organi-zatorov v kletkah embrio-nal'noy pochki svin'i (liniya SPEV) // Citologiya. - 2006. - T. 48, № 4. - S. 320-331.
4. Eliseev V.G., Afanas'ev Yu.I., Yurina N.A. Gistologiya. - M.: Medicina, 1983. - 590 s.
5. Zharskaya O.O., Zacepina O.V. Dinamika i mehanizmy reorganizacii yadryshka v mi-toze // Citologiya. - 2007. - Tom 49. - № 5. - S. 355-369.
6. Kroker Dzhen. Rayony yadryshkovogo organi-zatora i fibrillyarnye centry. Moleku-lyarnaya klinicheskaya diagnostika (metody) / pod red. S. Herringstona i Dzh. Makgi. - M.: Mir, 1999. - S. 260-279.
7. Mamaev N.N., Mamaeva S.E. Struktura i funkciya yadryshkoobrazuyuschih rayonov hro-mosom: molekulyarnye, citologicheskie i klinicheskie aspekty // Citologiya. - 1992. - T. 34, № 10. - S. 3-25.
8. Minina V.I., Savchenko Ya.A., Ahmat'yanova V.R. Vliyanie proizvodstvennoy sredy na funkcional'nuyu aktivnost' genov rDNK u rabochih salairskogo gorno-obogatitel'-nogo kombinata // Sovremennye problemy nauki i obrazovaniya. - 2006. - № 3. - S. 105.
9. Polikar A. Elementy fiziologii kletki. - L.: Nauka, 1976. - 389 s.
10. Robertis E., Novinskiy V., Saes F. Biolo-giya kletki. - M.: Mir, 1973. - 488 s.
11. Sabaneeva E.V. Specifichnost' okrashivaniya yadryshkovyh organizatorov azotnokislym serebrom // Citologiya. - 1989. - T. 31. - № 1. - S. 5-13.
12. Tashke K. Vvedenie v kolichestvennuyu cito-gistologicheskuyu morfologiyu / Akademiya socialisticheskoy respubliki Rumynii. - Buharest, 1980. - 192 s.
13. Chencov Yu.S. Vvedenie v kletochnuyu biolo-giyu. - Izd. 4-e, pererab. i dop. - M.: Aka-demkniga, 2004. - 495 s.
14. Yurko A.S., Kavcevich N.N. Rayony organi-zatorov yadryshka limfocitov gren-landskih tyuleney (Pagophilus groenlandicus Erxleben, 1777) raznogo vozrasta // Morskie mlekopitayuschie Golarktiki. - 2006. - S. 576-580.
15. Acta Neuropathol / Mc A.M. Nicol [et al.]. -1989. - V. 77, № 5. - P. 547-549.
16. Bauer NB, Zervos D, Moritz A. Argyrophilic nucleolar organizing regions and Ki67 equally reflect proliferation in fine needle aspirates of normal, hyperplastic, inflamed, and neoplastic canine lymph nodes (n = 101) // Journal of veterinary internal medicine (American college of Veterinary Internal Medicine). - 2007. - № 21 (5). - 928. - P. 35.
17. Cooper G.M. The Cell. A Molecular approach / 2nd ed. Sunderland (MA): Sinauer Associates, 2000. - 625 p.
18. Hernandez-Verdun D. The Nucleolus Today // J. Cell. Sci. - 1991. - V. 99. - P. 465-471.
19. Howell M., Black D.A. Controlled silverstaining of nucleolus organiser regions with a protec-tive colloidal developer. A 1-stepmethod // Experientia. - 1980. - № 36. - P. 1014-1015.
20. Nucleolar size indicates rapidity of cell prolif-eration in cancer tissues / M. Derenzini [et al.]. - 2000. Jun; 191 (2) 181-6.
21. Olson M.O.J. The role of proteins in Nucleolar Structure and function. In the eukariotic nucle-us. Molecular biochemistry and macromolecu-lar assemblies // V.2. Edited by Strauss P.R., Wilson S.H. Caldwell: The Telford Press. - 1990. - P. 519-559.
22. Sirri V., Roussel P., Hernandez-Verdun D. The AgNOR proteins: qualitative and quantitative changes during the cell cycle // Micron. - 2000. - V. 31 (2). - R. 121-126.
23. Sparrow A.H., Schairer L.A., Sparrow R.C. Re-lationship between nuclear volumes, chromo-some numbers and relative radiosensitivities // Sciece. - 1963. 163-6.