The Study of Free Radical Processes and Antioxidant Activity of Hybrid Composites with Hydroxyapatite on the Basis of Polystyrene In Vitro

N. Bagrovskaya; O. Alekseeva; S. Nazarov; Maya Klycheva; Olga Sitnikova; Irina Popova

doi:doi:10.12737/7285

The Study of Free Radical Processes and Antioxidant Activity of Hybrid Composites with Hydroxyapatite on the Basis of Polystyrene In Vitro

Published в Journal of New Medical Technologies · Volume 21, Issue 4, 2014 · Pages 129–133 · Rubrics: Medical Biophysics and Development of Treatment‐and‐Diagnostic Equipment

DOI 10.12737/7285

Received: 19.12.2014 Accepted: 19.12.2014 Published: 19.12.2014 Language of publication: RUS

Authors

N. Bagrovskaya · O. Alekseeva · S. Nazarov · Maya Klycheva · Olga Sitnikova · Irina Popova

ABSTRACT

The polystyrene composites, received by application polymeric solution (polystyrene) on teflon base sheet with the following samples drying at 293 K to constant mass were studied. The hydroxiapatite particles immobilization in polysterene matrix was made by mechanical dispersion. The polimeric’s systames hydroxiapatite concentration were 0,03; 0,1; 0,5 mac.%. The authors determined sorption and structural hydroxiapatite’s characteristics such as specific surface area and pore volume. The authors studied the influence of hydroxiapatite concentration in composites on lipid free radical processes and antioxidant activity of confluent blood serum and confluent serum after contact with composite, including hydroxiapatite in vitro. It was noted that polymeric composites with 0,1 and 0,5 mac% hydroxiapatite concentration increase the hemiluminescence parameters based on hemiluminescence analysis: fast flashes Imax and lightsum S. These data argue for free radical processes activation, so curve slope ratio increased tga and parameters A and Z decreased. It’s reflects the antioxidant activity increasing. The authors performed the investigation of malonic dialdehyde content and total blood serum antioxidant activity by spectrophotometry. The antioxidant activity increasing after composites impact, including 0.5mac% hydroxiapatite in confluent blood serum was determined. These data argue for composites’ antioxidant effects in comparison with control blood serum.

KEYWORDS

hydroxiapatite lipid free radical process antioxidant activity hemiluminescence.

Information References

Authors:

Type:

Article

Pages:

from 129 to 133

Status:

Published

Language:

Russian

Keywords:

hydroxiapatite, lipid free radical process, antioxidant activity, hemiluminescence.

References

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