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V stat'e rassmatrivaetsya vliyanie somaticheskogo ekstrakta Anisakis simplex L3 na kul'tury kletok mikroorganizmov in vitro. Ranee ustanovleno, chto pod deystviem ukazannogo ekstrakta narushaetsya i ugnetaetsya process deleniya eukarioticheskih kletok. Svedeniy o mehanizmah vzaimodeystviya somaticheskih ekstraktov gel'mintov i mikroorganizmov ochen' malo. Predpolagaetsya, chto somaticheskiy ekstrakt iz anizakid okazyvaet negativnoe vliyanie na mikroorganizmy za schet vhodyaschih v ego sostav belkovyh komponentov i metabolitov. Cel'yu issledovaniya yavlyalos' izuchenie vliyaniya ekstrakta na kul'tury kletok raznoobraznyh mikroorganizmov, kak po morfologicheskim priznakam, tak i po ustoychivosti k faktoram vneshney sredy. Ekstrakt gotovili iz lichinok anizakid, izvlechennyh iz zamorozhennoy putassu (Micromesistius poutassou), proveryali na steril'nost' i bezvrednost', opredelyali soderzhanie belka. Dlya issledovaniya ispol'zovali sutochnye kul'tury bakteriy: mikrokokki Micrococcus sp., palochki Escherichia coli, Proteus vulgaris, Salmonella tiphimurium i bacilly Bacillus subtilis. Pri kul'tivirovanii mikroorganizmov s diskami, propitannymi antigennym ekstraktom anizakid, v termostate pri +37 °S cherez 12 chasov vyyavlena zona zaderzhki rosta u Micrococcus sp., E. coli i P. vulgaris. Na rost bakteriy palochek S. tiphimurium, bacill B. subtilis ekstrakt vliyaniya ne okazyval. Formirovanie vyrazhennoy zony steril'nosti svidetel'stvuet o nalichii v sostave belkovogo ekstrakta biologicheski-aktivnyh komponentov, obladayuschih bakteriostaticheskim deystviem. Obsuzhdayutsya mehanizmy bakteriostaticheskogo deystviya somaticheskogo ekstrakta. Poluchennye v hode eksperimenta dannye podtverzhdayut antagonisticheskie otnosheniya belkovyh produktov lichinok Anisakis simplex L3 i mikroorganizmov, soglasuyuschihsya s literaturnymi dannymi. Vyyavlena razlichnaya stepen' aktivnosti antigenov somaticheskogo ekstrakta iz anizakid na kul'tury mikroorganizmov, naibol'shee vliyanie ekstrakt okazal na kul'tury palochek E. coli i mikrokokkov Micrococcus sp. Poluchennye rezul'taty mozhno ispol'zovat' dlya razrabotki modeli bakterial'nyh kletok kak test ob'ektov dlya ocenki antigennoy aktivnosti somaticheskih, ekskretorno-sekretornyh ekstraktov i metabolitov razlichnyh gel'mintov.
somaticheskiy ekstrakt, Anisakis simplex L3, metabolity, bakterii, bacilly, bakteriostaticheskoe deystvie.
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